bio rad cfx real time pcr instrument (Bio-Rad)
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Bio Rad Cfx Real Time Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 15377 article reviews
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Real-time Polymerase Chain Reaction:Article Title: Enhanced B cell electroporation efficiency via inhibition of DNA-induced apoptosis and pyroptosis with pan-caspase inhibitor Article Snippet: .. Afterward, the qPCR mixture containing Master Mix and ligase was added, and the qPCR assay was performed on a Article Title: Leuconostoc citreum enhances the colonization ability of Lactiplantibacillus plantarum in the gut by synergistically modifying the intestinal niche. Article Snippet: .. After extracting and purifying fecal DNA for use as a template, PCR amplification and detection were carried out using Lp082specific primers (forward: 5’-GCCTTGAACTTTG TGCCTGTC-3’; reverse: 5’-GGCTTTGCCTGT TGATGCTTA-3’) on a Article Title: Antimicrobial Resistance Gene Patterns in Traditional Montenegrin Njeguški Cheese Revealed by qPCR Article Snippet: .. Assays were run on a Article Title: Polycomb repression works without Siesta Article Snippet: .. Serial dilutions of genomic DNA were used to make the standard curve. qPCR analysis was performed using a BioRad Article Title: Enhanced B cell electroporation efficiency via inhibition of DNA-induced apoptosis and pyroptosis with pan-caspase inhibitor. Article Snippet: .. Afterward, the qPCR mixture containing Master Mix and ligase was added, and the qPCR assay was performed on a Article Title: Synergistic neurotoxicity of polystyrene nanoparticles and MEHP in zebrafish (Danio rerio). Article Snippet: The surge in global plastic usage has exacerbated the pollution of nanoplastics and plasticizers in aquatic ecosystems, causing widespread concern about population health issues.. We used a zebrafish model to investigate the combined toxicity of polystyrene nanoparticles (PS-NPs) and plasticizer metabolite mono-(2-ethylhexyl) phthalate (MEHP), revealing the synergistic neurotoxic effects and potential mechanisms of compound exposure.. Co-exposure amplifies the bioaccumulation of PS-NPs (p < 0.05), causing innate immune damage (p < 0.0001), severe oxidative stress (p < 0.0001), and embryonic apoptosis (p < 0.05), while inducing larval neuromotor dysfunction and changes in neural markers. Article Title: The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells Article Snippet: The purity and concentration of RNA were determined using a nanodrop spectrophotometer (NanoDrop; Thermo Fisher Scientific, Massachusetts, USA), and first-strand cDNA synthesis was performed using RNA from lysed cultured cells and iScriptTM reverse transcriptase kit (Bio-Rad, California, USA). .. The qPCR reactions were carried out using a Article Title: Disruption of FW2.2-like Genes Enhances Metallic Micronutrient Accumulation in Brown Rice Article Snippet: Total RNA (0.8 μg) was reverse-transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Dalian, China). .. PCR amplification was conducted using TB Green Premix Ex Taq II (Takara) in a Polymerase Chain Reaction:Article Title: Leuconostoc citreum enhances the colonization ability of Lactiplantibacillus plantarum in the gut by synergistically modifying the intestinal niche. Article Snippet: .. After extracting and purifying fecal DNA for use as a template, PCR amplification and detection were carried out using Lp082specific primers (forward: 5’-GCCTTGAACTTTG TGCCTGTC-3’; reverse: 5’-GGCTTTGCCTGT TGATGCTTA-3’) on a Article Title: Disruption of FW2.2-like Genes Enhances Metallic Micronutrient Accumulation in Brown Rice Article Snippet: Total RNA (0.8 μg) was reverse-transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Dalian, China). .. PCR amplification was conducted using TB Green Premix Ex Taq II (Takara) in a Amplification:Article Title: Leuconostoc citreum enhances the colonization ability of Lactiplantibacillus plantarum in the gut by synergistically modifying the intestinal niche. Article Snippet: .. After extracting and purifying fecal DNA for use as a template, PCR amplification and detection were carried out using Lp082specific primers (forward: 5’-GCCTTGAACTTTG TGCCTGTC-3’; reverse: 5’-GGCTTTGCCTGT TGATGCTTA-3’) on a Article Title: Antimicrobial Resistance Gene Patterns in Traditional Montenegrin Njeguški Cheese Revealed by qPCR Article Snippet: .. Assays were run on a Article Title: Disruption of FW2.2-like Genes Enhances Metallic Micronutrient Accumulation in Brown Rice Article Snippet: Total RNA (0.8 μg) was reverse-transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Dalian, China). .. PCR amplification was conducted using TB Green Premix Ex Taq II (Takara) in a SYBR Green Assay:Article Title: The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells Article Snippet: The purity and concentration of RNA were determined using a nanodrop spectrophotometer (NanoDrop; Thermo Fisher Scientific, Massachusetts, USA), and first-strand cDNA synthesis was performed using RNA from lysed cultured cells and iScriptTM reverse transcriptase kit (Bio-Rad, California, USA). .. The qPCR reactions were carried out using a |
